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KMID : 1191520110160020025
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Journal of Korean Traditional Oncology
2011 Volume.16 No. 2 p.25 ~ p.34
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Inhibition of LPS-induced Inflammatory Biomarkers by Fraction of Citrus hassaku pericarp through Suppression of NF- Activation in RAW264.7 Cells
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Kim Chul-Won
Kim Sung-moo
Jeong Seung-Weon
Kim So-Mi
Ahn Kwang-Seok
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Abstract
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Objectives : Citrus is the fruit that is readily available around us. Therefore, we investigated the anti-inflammatory effects of fraction isolated from the Citrus hassaku pericarp in RAW264.7 macrophage cells.
Methods : The effects of fraction from Citrus hassaku pericarp on cell viability on RAW264.7 cells were measured by the MTT assay. The mRNA levels of iNOS and COX-2, its protein level by fraction of Citrus hassaku pericarp treatment in RAW264.7 macrophage cells were investigated by RT-PCR and immunoblots. Nitrite accumulation in the culture was measured colorimetrically by the Griess reaction using a Griess reagent. The amount of IL-6 and TNF- production was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
Results : The results indicated that the fraction of Citrus hassaku pericarp concentration highly suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) and IL-6 productions without a cytotoxic effect on RAW264.7 cells. fraction of Citrus hassaku pericarp inhibited the expressions of LPS-induced iNOS and COX-2 protein and their mRNA in a dose-dependent manner. Particularly, fraction of Citrus hassaku pericarp suppressed the level of nuclear factor- (NF-) activity, which was linked with the suppression of LPS-induced phosphorylation of p65 at serine 276 and p65 translocation into nuclei, but not MAPK signaling. In addition, treatment with fraction of Citrus hassaku pericarp inhibited the production of IL-6 and TNF- in LPS-stimulated RAW264.7 cells.
Conclusion : Our results indicate that fraction of Citrus hassaku pericarp potentially inhibits the biomarkers related to inflammation through the blocking of NF- p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.
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KEYWORD
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fraction of Citrus hassaku pericarp, inflammation, NF-, nitric oxide, cyclooxygenase-2
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